9 research outputs found
Spatial genetic structure of Thai silver barb Puntius gonionotus (bleeker) populations in Thailand
ThesisThesis, Dalhousie University, 199
Manual on application of molecular tools in aquaculture and inland fisheries management. Part 2. Laboratory protocols and data analysis
The aim of this manual is to provide a comprehensive practical tool for the generation and analysis of genetic data for subsequent application in aquatic resources management in relation to genetic stock identification in inland fisheries and aquaculture.
The material only covers general background on genetics in relation to aquaculture and fisheries resource management, the techniques and relevant methods of data analysis that are commonly used to address questions relating to genetic resource characterisation and population genetic analyses. No attempt is made to include applications of genetic improvement techniques e.g. selective breeding or producing genetically modified organisms (GMOs). The manual includes two ‘stand-alone’ parts, of which this is the second volume:
Part 1 – Conceptual basis of population genetic approaches: will provide a basic foundation on genetics in general, and concepts of population genetics. Issues on the choices of molecular markers and project design are also discussed.
Part 2 – Laboratory protocols, data management and analysis: will provide step-by-step protocols of the most commonly used molecular genetic techniques utilised in population genetics and systematic studies. In addition, a brief discussion and explanation of how these data are managed and analysed is also included.
This manual is expected to enable NACA member country personnel to be trained to undertake molecular genetic studies in their own institutions, and as such is aimed at middle and higher level technical grades. The manual can also provide useful teaching material for specialised advanced level university courses in the region and postgraduate students. The manual has gone through two development/improvement stages. The initial material was tested at a regional workshop and at the second stage feedback from participants was used to improve the contents
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Session D5: Efficiency of Fish Passages Facilities on Nam Kam River, Thailand
Abstract:
Distribution and migration of fishes in Nam Kam River, one of the Mekong tributary in Thailand, were conducted during the migration period in 2013. Nam Kam River consists of four cascade watergates, all of which equipped with fish passage. Migration of fish from the Mekong River into the Nam Kam river system started at the onset of the rainy season around the end of May to early October.
It was found that 92 species from 21 families were observed in the fish passages. The highest number (440,015 fish) was found at the most downstream passage consisting of 83 species. Maximum number of fish was found in early rainy season with averaging 46,831 individuals per day. Fish migrated during daytime more than nighttime in term of abundance (93.03-95.80% vs 4.18- 6.97%) while number of species was not significantly difference. Cyprinids was the dominant group migrated during daytime while Bagrids and Silurids were mostly migrated at nighttime. Majority of fishes were mature and ready to spawn, indicated the reproductive migration.
Distribution after migrating through fish passage using physical and genetic (microsatellites) tags were studied in two economically important species (Hemibagrus nemurus and Osteochilus hasselti). The recapture data revealed target species could migrate through a few watergates upstream at the farthest distance of 72.2 km from most downstream watergate. While the microsatellite analysis indicated that fishes could migrate, although at limited number, almost 100 km upstream. Tag recovery from physical and genetic tagging technique were very low (0.62- 10.05% and 0.95% in H. nemurus and 0.75- 0.92% and 0.58% in O. hasselti) as well as the effective numbers of migrants (2.7426 in H. nemurus and 8.2676 in O. hasselti). The result indicated that a series of watergates might, to some extent, obstruct the migration of fishes in Nam Kam River. Management of watergates based on the migrating period and spawning season could increase the rate of fish migration in the river
Effects of intraspecific competition on genetic parameter estimates of size at age and size specific growth in Tilapia
ThesisThesis, in partial fulfillment of the requirements for the degree of Master of Science, Dalhousie University, 198
Manual on application of molecular tools in aquaculture and inland fisheries management. Part I : conceptual basis of population genetic approaches
The aim of this manual is to provide a comprehensive practical tool for the generation and analysis of genetic data for subsequent application in aquatic resources management in relation to genetic stock identification in inland fisheries and aquaculture. The material only covers general background on genetics in relation to aquaculture and fisheries resource management, the techniques and relevant methods of data analysis that are commonly used to address questions relating to genetic resource characterisation and population genetic analyses. No attempt is made to include applications of genetic improvement techniques e.g. selective breeding or producing genetically modified organisms (GMOs). The manual includes two ‘stand-alone’ parts, of which this is the first volume: Part 1 – Conceptual basis of population genetic approaches: will provide a basic foundation on genetics in general, and concepts of population genetics. Issues on the choices of molecular markers and project design are also discussed. Part 2 – Laboratory protocols, data management and analysis: will provide step-by-step protocols of the most commonly used molecular genetic techniques utilised in population genetics and systematic studies. In addition, a brief discussion and explanation of how these data are managed and analysed is also included. This manual is expected to enable NACA member country personnel to be trained to undertake molecular genetic studies in their own institutions, and as such is aimed at middle and higher level technical grades. The manual can also provide useful teaching material for specialised advanced level university courses in the region and postgraduate students. The manual has gone through two development/improvement stages. The initial material was tested at a regional workshop and at the second stage feedback from participants was used to improve the contents.</div
Manual on application of molecular tools in aquaculture and inland fisheries management. Part I : laboratory protocols, data management and analysis
The aim of this manual is to provide a comprehensive practical tool for the generation and analysis of genetic data for subsequent application in aquatic resources management in relation to genetic stock identification in inland fisheries and aquaculture. The material only covers general background on genetics in relation to aquaculture and fisheries resource management, the techniques and relevant methods of data analysis that are commonly used to address questions relating to genetic resource characterisation and population genetic analyses. No attempt is made to include applications of genetic improvement techniques e.g. selective breeding or producing genetically modified organisms (GMOs). The manual includes two ‘stand-alone’ parts, of which this is the second volume: Part 1 – Conceptual basis of population genetic approaches: will provide a basic foundation on genetics in general, and concepts of population genetics. Issues on the choices of molecular markers and project design are also discussed. Part 2 – Laboratory protocols, data management and analysis: will provide step-by-step protocols of the most commonly used molecular genetic techniques utilised in population genetics and systematic studies. In addition, a brief discussion and explanation of how these data are managed and analysed is also included. This manual is expected to enable NACA member country personnel to be trained to undertake molecular genetic studies in their own institutions, and as such is aimed at middle and higher level technical grades. The manual can also provide useful teaching material for specialised advanced level university courses in the region and postgraduate students. The manual has gone through two development/improvement stages. The initial material was tested at a regional workshop and at the second stage feedback from participants was used to improve the contents.</div